(1)提取线粒体分离介质:准确称取蔗糖42.79g,EDTA 0.0736g,HCl 0.285ml,Tris 0.6057g,加蒸馏水至500ml,充分混匀。 (2)配细胞裂解液:准确称取Hepes 238.4mg(10mmol/L),KCl 74.5mg(10mmol/L),EDTA 2.9mg(0.1mmol/L),EGTA 3.8mg(0.1mmol/L),DTT 7.7mg(临用时加1mol/L DTT 49.8 l,0.5mmol/L),PMSF 3.4mg(临用时加100mmol/L PMS ......