PKasewasdialyzedagainst10mM,pH7.0phosphatebufferforseveralhoursa℃,and thenastockenzymesolutionwasmadew ﹒ Thefi nalenzymeconcentrationwas5.5mg/mL ﹒ Theadditiveconcentrationsusedint were3.0mgdisaccharides(trehalose。Residualwatercontentofthelyophil pleswhent ......